Melatonin ameliorates testicular damages induced by nicotine in mice

Authors

  • Sina Khajeh Jahromi Cellular & Molecular Research Center, Guilan University of Medical Sciences, Rasht, Iran
Abstract:

Objective(s): Nicotine as a toxic substance leads to impairment of the reproductive system function. The aim of this study was to evaluate effects of melatonin on testicular alterations, sperm nuclear integrity, and epididymal sperm parameters in mice treated with nicotine.Materials and Methods: Male mice were divided into four groups. Group A received the vehicle, group B received nicotine 0.1 mg/100 g BW, group C received melatonin 10 mg/kg, group D received nicotine plus melatonin. Evaluations were made by histology and Johnson’s score for study of spermatogenesis, immunostaining for  study of  male germ cells apoptosis, sperm chromatin dispersion (SCD) test for assaying sperm chromatin integrity, enzyme-linked immunosorbent assay (ELISA) for assessment of serum levels of testosterone and luteinizing hormone (LH), and sperm parameters including morphology, motility, and count. Results: Nicotine caused a significant decrease in spermatogenesis quantity and Johnson’s score, sperm parameters, and sex hormones. Melatonin in group D, increased sperm chromatin integrity, improved spermatogenesis, Johnson’s score, and sperm parameters (P

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Journal title

volume 21  issue 6

pages  639- 644

publication date 2018-06-01

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